Analysis Note

ControlJurkat and Raji cells, tonsil tissue

Application

Anti-PECAM-1 Antibody, clone 390 is an antibody against PECAM-1 for use in FC, IH & IP.

Flow Cytometry: 1 µg/10e6 cells.

Immunohistochemistry: acteone fixed frozen tissue sections

Immunoprecipitation

Blocking of cell adhesion: CBL1337 inhibits aggregation of L cells transfected with a variant form of CD31 (DeLisser, 1993)

Optimal working dilutions must be determined by the end user.

General description

CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1) is a type I integral membrane glycoprotein, and a member of the immunoglobulin superfamily of cell surface receptors. PECAM-1 is constitutively expressed on the surface of endothelial cells where it is concentrated at cell junctions. PECAM-1 is also expressed at low levels on all leukocytes and platelets. Homotypic interaction of CD31 is important in cell-cell interaction, cell-matrix interaction and signal transduction.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A Purified mouse immunoglobulin in 100mM borate buffered saline, pH 8.0, containing no preservative.

Format: Purified